RNA单分子直接测序

RNA结构图.jpg我们对于人体生理和疾病的最终认知取决于对于人类基因组以及其功能的完整理解。现在常用的转录组分析方法都是非直接的,需要把RNA转变为cDNA,甚至于在cDNA合成的过程中也参入了包括合理的特性描述和转录定量的人工干预。而且cDNA合成不适合于对小片段,降解的或者微量RNA样品。RNA直接测序则不需要经过RNA到cDNA转化,而是可以直接进行单分子RNA测序的方法。因此RNA直接测序可以更准确的定量RNA表达,实现了通过高通量,低成本的直接RNA测序方案达到可以综合,无偏差的研究转录组学的目的。

 


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RNA传统方法测序:通过RNA先反转录成cDNA,然后对cDNA进行测序。应用领域,转录组定量,小RNA测序。

RNA直接测序(研发方向):无需将RNA反转录成cDNA,直接对RNA进行测序。同时也可以做常规RNA直接测序和靶向RNA直接测序。

 








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